Ballpark Dispensary in conjunction with RM3 Labs did a potency and terpene study on Green Crack and have concluded that Green Crack is a cannabis strain that helps with PTSD.
Total Terpenes = 1.46%
According to Russo (2011), cannabis terpenoids share precursors with phytocannabinoids and consist of flavor and fragrance components. The table below adapted from Russo (2011) lists some of these terpenoids, common natural sources, and pharmacological activity.
|Terpenoid||Common Source||Potential Benefits|
Possible addiction treatment
|Limonene||Lemon||Boosts immune system |
Apoptosis of breast cancer cells
Active against acne bacteria
Sedating, muscle relaxant, hypnotic
|Nerolidol||Orange||Sedative via inhalation on mice |
Rm3 Labs Terpene Testing Methods
At Rm3 Labs we use a testing methodology known as Headspace Gas-Chromatography with Flame Ionization Detection, or headspace GC-FID. This method is widely used in the environmental and pharmaceutical industries to analyze for product or environmental contamination. For each test, the client provides us a small sample of the product. We heat the sample in an airtight vial to vaporize the terpenes, sample the headspace in the vial and inject this headspace sample into the gas chromatograph for chemical analysis. In analyzing sample headspace, we screen out various matrix interferences present in the concentrate. “ND” = not detected or below our limit of accurate detection or limit of quantification for our test.
THC = 26%
*Inactive, acidic forms of the THC, CBD and CBG molecules, which convert to the active forms given time and/or heat. Acidic counts are adjusted to account for the greater weight of the acidic molecules.
** This shows the amount of initial THC-A converted to THC by the time of testing. At harvest, this number is near zero. For well-cured plant material, this figure generally ranges from 5% to 20%. For edibles and tinctures, this number should be over 85%. (This is for marijuana only, not industrial hemp)
N/D = Not Detected; below our limit of accurate quantitation for the test.
< LOQ = Values below the limit of quantitation are outside the range of accuracy.
The Biochemistry of Marijuana
The major active components of marijuana are a family of compounds known as cannabinoids. There are over 60 different identified cannabinoids, though only a handful have been closely studied; as a family, they have remarkable medical properties. Cannabinoids have been shown to have widely varied effects, including psychoactivity, pain relief, nausea relief, anti-inflammatory, anti-bacterial and anti-cancer effects.
Cannabinoids are effective because they mimic natural neurotransmitters – _the chemical messengers between cells – _that act in the human brain and throughout the body. Different cannabinoids interact with the body in complex ways. Some compounds enhance or inhibit the effects of others, or help alleviate the side effects that one compound alone might cause. The mix of different cannabinoids in a particular strain of marijuana are largely governed by the plant’s genetics and maturity at harvest, while the total levels of cannabinoids are dictated both by genetics and the overall quality of the growing conditions.
THC, or delta-9 tetrahydrocannabinol, is the primary psychoactive component of marijuana. It is responsible for the high of marijuana, and is likely the direct or indirect cause of unwanted side effects such as anxiety, detachment and paranoia. When marijuana is harvested, THC is primarily present in its “inactive”, acidic form, known as THC-A, which has little psychoactive effect. As marijuana cures, the THC-A partially converts to THC; the conversion is accelerated as marijuana is heated during cooking or smoking.
CBD, or cannabidiol, is the second most prominent and studied cannabinoid, and is the major cannabinoid in “hemp-type” marijuana. CBD has many effects in itself, and it also appears to alleviate many of the side effects of THC, including sleepiness and loss of memory.
CBN, or cannabinol, is the breakdown product of THC; high levels indicate that the marijuana may be relatively old or has been stored at high temperatures.
Research has demonstrated these properties, among others, of the different cannabinoids:
*Includes cannabichromene (CBC), cannabigerol (CBG) and tetrahydrocannabivarin (THCV).
Rm3’s Testing Methods
At Rm3 Labs we use a testing methodology known as High-Performance Liquid Chromatography, or HPLC. This method is widely used in the herbal, nutritional supplement and pharmaceutical industries to ensure the purity and strength of products. For each test, the client provides us a small sample of the product. We extract the cannabinoids from the sample, and then separate the individual components. Analysis of the separated components is done by computer.
In analyzing samples, we screen out seeds and large stems, as including them in the sample may produce large variations in results. Cannabinoids are measured as a percentage of dry weight, to eliminate the effect of particularly moist storage conditions on the sample. End users should inspect the product they receive for excessive seeds, stems or moisture levels.
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